Mouse CD68 Polyclonal antibody, PBS Only

CD68 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30929-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 30929-1-AP (same clone as 30929-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30929-1-AP (CD68 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 30929-1-AP (same clone as 30929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (30929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 30929-1-AP (same clone as 30929-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (30929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30929-1-PBS in a different storage buffer formulation.

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Product Information

30929-1-PBS targets CD68 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD68 antigen
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: NM_001291058.1
• Gene Symbol: Cd68
• Gene ID (NCBI): 12514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.