CD64 Recombinant antibody

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  WB analysis using 84518-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84518-2-RR (CD64 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 84518-2-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD64/FCGR1 antibody (84518-2-RR, Clone: 241809D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84518-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84518-2-RR against Mouse CD64 were performed. The affinity constant is 74.4 pM.

CD64 Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84518-2-RR

Clone No.241809D6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

High affinity immunoglobulin gamma Fc receptor I, FcRI, Fcgr1, Fc-gamma RI, Fcg1

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, J774A.1 cells
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84518-2-RR targets CD64 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: Fc receptor, IgG, high affinity I
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 68-72 kDa
• GenBank Accession Number: NM_010186.5
• Gene Symbol: Fcgr1
• Gene ID (NCBI): 14129
• RRID: AB_3672024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P26151
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.