CD6 Recombinant antibody, PBS Only (Detector)

CD6 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241965B11

Cat no : 84508-2-PBS

Synonyms

TP120, T-cell differentiation antigen CD6, T12, Soluble CD6, CD6 molecule

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01358-2

  Sandwich ELISA standard curve of MP01358-2, Human CD6 Recombinant Matched Antibody Pair - PBS only. 84508-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2079. 84508-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP01358-2

  The mean CD6 concentration was determined to be 20.8 ng/mL in MOLT-4 cell extract based on a 1.2 mg/mL extract load and 10.9 ng/mL in Jurkat cell extract based on a 4.2 mg/mL extract load.

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  Sample test of MP01358-2

  Serum of six individual healthy human donors was measured. The CD6 concentration of detected samples was determined to be 2,345.6 pg/mL with a range of 1,106.3-6,008.2 pg/mL.

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  Cytometric bead array standard curve of MP01358-2

  Cytometric bead array standard curve of MP01358-2, CD6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84508-1-PBS. Detection antibody: 84508-2-PBS. Standard: Eg2079. Range: 0.781-100 ng/mL.

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Product Information

84508-2-PBS targets CD6 as part of a matched antibody pair:

MP01358-2: 84508-1-PBS capture and 84508-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD6 molecule
• GenBank Accession Number: BC033755
• Gene Symbol: CD6
• Gene ID (NCBI): 923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD6 is a type I transmembrane glycoprotein of 105-130 kDa that belongs to the scavenger receptor cysteine-rich superfamily (PMID: 21880988). It is composed of an extracellular region, which consists of three tandem SRCR domains, a transmembrane region, and a cytoplasmic tail devoid of intrinsic catalytic activity but harbouring several phosphorylatable residues suitable for intracellular signal transduction (PMID: 38139340; 23711376). CD6 is expressed by T cells, medullary thymocytes, a subset of B cells and NK cells, and in some cells of the brain (PMID: 1919444; 21178331). CD6 plays a role in lymphocyte activation, proliferation, and survival processes via interaction with its endogenous ligands.