Anti-Human CD59 Rabbit Recombinant Antibody

CD59 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241311A4

Cat no : 98099-1-RR

Synonyms

HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 241311A4

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Validation Data Gallery

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  FC experiment of human PBMCs using 98099-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD59 Rabbit Recombinant Antibody (98099-1-RR, Clone:241311A4) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG, Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98099-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD59 Rabbit Recombinant Antibody (98099-1-RR, Clone:241311A4) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98099-1-RR

  1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human CD59 Rabbit Recombinant Antibody (98099-1-RR, Clone:241311A4) and PE-conjugated Goat Anti-Rabbit IgG, and 0.25 ug APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3), and 0.25 ug Isotype Control. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98099-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98099-1-RR against Human CD59 were performed. The affinity constant is 0.888 nM.

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Tested Applications

• Positive FC detected in: FC receptor blocked human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98099-1-RR targets CD59 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD59 molecule, complement regulatory protein
• Calculated Molecular Weight: 19 kDa
• GenBank Accession Number: BC001506
• Gene Symbol: CD59
• Gene ID (NCBI): 966
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.