1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD58 (65193-1-Ig, Clone:TS2/9) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD58 (65193-1-Ig, Clone:TS2/9) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.
FC experiment of human PBMCs using 65193-1-Ig
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD58 (65193-1-Ig, Clone:TS2/9) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD58 (65193-1-Ig, Clone:TS2/9) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
65193-1-Ig targets CD58 in FC applications and shows reactivity with Human samples.
Store at 2-8°C. Stable for one year after shipment.
Background Information
CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD58 (65193-1-Ig, Clone:TS2/9) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.
FC experiment of human PBMCs using 65193-1-Ig
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD58 (65193-1-Ig, Clone:TS2/9) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD58 Monoclonal antibody
Catalog Number
65193-1-Ig
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
