CD58 Polyclonal antibody

CD58 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10878-1-AP

Synonyms

Surface glycoprotein LFA 3, Lymphocyte function-associated antigen 3, LFA3, LFA 3, CD58 molecule

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Validation Data Gallery

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  WB analysis using 10878-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 10878-1-AP (CD58 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of TF-1 using 10878-1-AP

  TF-1 cells were subjected to SDS PAGE followed by western blot with 10878-1-AP (CD58 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10878-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10878-1-AP (CD58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lymph using 10878-1-AP

  human lymph tissue were subjected to SDS PAGE followed by western blot with 10878-1-AP (CD58 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 10878-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10878-1-AP (CD58 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 10878-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10878-1-AP (CD58 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 10878-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10878-1-AP (CD58 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Raji cells, Jurkat cells, TF-1 cells, HeLa cells, human lymph tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10878-1-AP targets CD58 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD58 fusion protein Ag1165
• Full Name: CD58 molecule
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 55-70 kDa
• GenBank Accession Number: BC005930
• Gene Symbol: CD58
• Gene ID (NCBI): 965
• RRID: AB_2260179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD58 a heavily glycosylated protein of 55-70 kDa, is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells. The protein is localized to the plasma membrane.

Publications

Species	Application	Title
human	WB	Heliyon; Clinical significance of immune-related antigen CD58 in gliomas and analysis of its potential core related gene clusters; Authors - Zhi Tian