PE Anti-Human CD56 (MEM 188)

CD56 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

MEM-188

Cat no : PE-65067

Synonyms

CD56, MSK39, N CAM 1, NCAM, NCAM 1, NCAM1

---

Validation Data Gallery

• 

  FC experiment of human peripheral blood lymphocytes using PE-65067

  1X10^6 human peripheral blood lymphocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Human CD3 (CL647-65151, Clone:UCHT1) and 0.2 ug PE Anti-Human CD56 (PE-65067, Clone: MEM 188). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using PE-65067

  1X10^6 human peripheral blood lymphocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Human CD3 (CL647-65151, Clone:UCHT1) and 0.2 ug PE-Mouse IgG2a Isotype Control. Cells were not fixed.

---

Tested Applications

• Positive FC detected in: human peripheral blood lymphocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65067 targets CD56 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Acute myelogenous leukemia cell line KG-1
• Full Name: neural cell adhesion molecule 1
• GenBank Accession Number: BC014205
• Gene Symbol: NCAM1
• Gene ID (NCBI): 4684
• RRID: AB_2883864
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and a stabilizer.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas.