CoraLite® Plus 647 Anti-Human ICAM-1/CD54 (15.2) Mouse IgG1 Recombinant Antibody

ICAM-1/CD54 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

15.2

Cat no : CL647-65544

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

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Validation Data Gallery

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  FC experiment of human PBMCs using CL647-65544

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD54 Mouse Recombinant Antibody (CL647-65544, Clone:15.2) or CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21), and 5 ul FITC Plus Anti-Human CD3 (OKT3) (FITC-65133, Clone: OKT3). Cells were not fixed. Lymphocytes and monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65544 targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Recombinant
• Type: Antibody
• Immunogen: Rheumatoid synovial cells and human monocytes
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 90 kDa
• GenBank Accession Number: BC015969
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 3383
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.