Anti-Human ICAM-1/CD54 (15.2) Mouse IgG2a Recombinant Antibody, PBS Only

ICAM-1/CD54 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

15.2

Cat no : 65567-1-PBS

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

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Validation Data Gallery

• 

  FC experiment of human PBMCs using 65567-1-MR (same clone as 65567-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD54 Mouse IgG2a Recombinant Antibody (65567-1-MR, Clone:15.2) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65567-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65567-1-PBS

  1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD54 (15.2) Mouse Recombinant Antibody, PBS Only (65567-1-PBS, Clone:15.2), or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Cells were then stained with APC Anti-Human CD3 (OKT3) (APC-65133, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65567-1-MR (same clone as 65567-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD54 Mouse IgG2a Recombinant Antibody (65567-1-MR, Clone:15.2) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65567-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65567-1-PBS

  1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD54 (15.2) Mouse Recombinant Antibody, PBS Only (65567-1-PBS, Clone:15.2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (right), or 0.125 ug Anti-Human CD54 (ICAM-1) (15.2) (65075-1-Ig, Clone: 15.2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (left). Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65567-1-MR (same clone as 65567-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD54 Mouse IgG2a Recombinant Antibody (65567-1-MR, Clone:15.2) (left) or 0.25 ug Anti-Human CD54 (ICAM-1) (65075-1-Ig, Clone: 15.2) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65567-1-PBS in a different storage buffer formulation.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65567-1-PBS targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Rheumatoid synovial cells and human monocytes
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 90 kDa
• GenBank Accession Number: BC015969
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 3383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).