Anti-Human CD5 Rabbit Recombinant Antibody

CD5 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240428A12

Cat no : 98054-1-RR

Synonyms

T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule

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Validation Data Gallery

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  FC experiment of human PBMCs using 98054-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Rabbit Recombinant Antibody (98054-1-RR, Clone: 240428A12) or 0.25 ug Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98054-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Rabbit Recombinant Antibody (98054-1-RR, Clone: 240428A12) (red) or 0.25 ug Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated.

• 

  Affinity and Kinetic Characterization of 98054-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98054-1-RR against Human CD5 were performed. The affinity constant is 0.27 nM.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98054-1-RR is CD5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD5 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: NM_014207.4
• Gene Symbol: CD5
• Gene ID (NCBI): 921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).