CD5 Recombinant antibody, PBS Only

CD5 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240428G2

Cat no : 83543-7-PBS

Synonyms

T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule

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Validation Data Gallery

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  WB analysis of Jurkat using 83543-7-RR (same clone as 83543-7-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 83543-7-RR (CD5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83543-7-RR (same clone as 83543-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83543-7-RR (same clone as 83543-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 83543-7-RR (same clone as 83543-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 83543-7-RR (same clone as 83543-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83543-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83543-7-RR against Human CD5 were performed. The affinity constant is 2.09 nM.

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Product Information

The immunogen of 83543-7-PBS is CD5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD5 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: NM_014207.4
• Gene Symbol: CD5
• Gene ID (NCBI): 921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.