Tested Applications
Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83543-6-RR targets CD5 in IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Fusion Protein Predict reactive species |
Full Name | CD5 molecule |
Calculated Molecular Weight | 55 kDa |
GenBank Accession Number | NM_014207.4 |
Gene Symbol | CD5 |
Gene ID (NCBI) | 921 |
ENSEMBL Gene ID | ENSG00000110448 |
RRID | AB_3671162 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P06127 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).
Protocols
Product Specific Protocols | |
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IF protocol for CD5 antibody 83543-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |