CD5 Recombinant antibody

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  IF Staining of Jurkat using 83543-6-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji and Jurkat cells using CD5 antibody (83543-6-RR, Clone: 240428A12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  Affinity and Kinetic Characterization of 83543-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83543-6-RR against Human CD5 were performed. The affinity constant is 0.747 nM.

CD5 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Cat No. 83543-6-RR

Clone No.240428A12

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Synonyms

T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule

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Tested Applications

• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83543-6-RR targets CD5 in IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD5 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: NM_014207.4
• Gene Symbol: CD5
• Gene ID (NCBI): 921
• ENSEMBL Gene ID: ENSG00000110448
• RRID: AB_3671162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P06127
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).