Anti-Human CD5 (L17F12) Mouse IgG2a Recombinant Antibody, PBS Only

• 

  FC experiment of human PBMCs using 65599-1-MR (same clone as 65599-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Mouse IgG2a Recombinant Antibody (65599-1-MR, Clone: L17F12) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65599-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65599-1-MR (same clone as 65599-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Mouse IgG2a Recombinant Antibody (65599-1-MR, Clone:L17F12) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65599-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65599-1-MR (same clone as 65599-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Mouse IgG2a Recombinant Antibody (65599-1-MR, Clone:L17F12) (left) or 0.25 ug Anti-Human CD5 (65200-1-Ig, Clone: L17F12) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65599-1-PBS in a different storage buffer formulation.

CD5 Recombinant Antibody for FC

Cat No. 65599-1-PBS

Clone No.L17F12

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

---

Product Information

65599-1-PBS targets CD5 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human T-cell acute lymphoblastic leukemia (T-ALL) cells
• Full Name: CD5 molecule
• Calculated Molecular Weight: 495 aa, 55 kDa
• GenBank Accession Number: BC027901
• Gene Symbol: CD5
• Gene ID (NCBI): 921
• ENSEMBL Gene ID: ENSG00000110448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).