Anti-Human CD5 (L17F12) Mouse IgG1 Recombinant Antibody, PBS Only

CD5 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

L17F12

Cat no : 65530-1-PBS

Synonyms

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1

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Validation Data Gallery

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  FC experiment of human PBMCs using 65530-1-PBS

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-PBS, Clone:L17F12) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65530-1-PBS

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-PBS, Clone:L17F12) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65530-1-PBS

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-PBS, Clone:L17F12) (left) or with 0.1 ug Anti-Human CD5 Mouse Monoclonal Recombinant Antibody (Clone:L17F12) (center and right) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65530-1-PBS targets CD5 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Recombinant
• Type: Antibody
• Immunogen: Human T-cell acute lymphoblastic leukemia (T-ALL) cells
• Full Name: CD5 molecule
• Calculated Molecular Weight: 495 aa, 55 kDa
• GenBank Accession Number: BC027901
• Gene Symbol: CD5
• Gene ID (NCBI): 921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).