1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65513-1-RR (same clone as 65513-1-PBS)
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-RR, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65513-1-PBS in a different storage buffer formulation.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-RR, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65513-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65513-1-RR (same clone as 65513-1-PBS)
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-RR, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65513-1-PBS in a different storage buffer formulation.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-RR, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65513-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65513-1-PBS
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65513-1-PBS
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000 (left and red), or stained with 0.2 ug Anti-Human CD5 Mouse Monoclonal Antibody (65152-1-Ig, Clone:UCHT2) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000 (center and green). Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000 (left and red), or stained with 0.2 ug Anti-Human CD5 Mouse Monoclonal Antibody (65152-1-Ig, Clone:UCHT2) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000 (center and green). Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65513-1-RR (same clone as 65513-1-PBS)
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-RR, Clone:UCHT2) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000 (left and red), or stained with 0.2 ug Anti-Human CD5 Mouse Monoclonal Antibody (65152-1-Ig, Clone:UCHT2) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000 (center and green). Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65513-1-PBS in a different storage buffer formulation.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-RR, Clone:UCHT2) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000 (left and red), or stained with 0.2 ug Anti-Human CD5 Mouse Monoclonal Antibody (65152-1-Ig, Clone:UCHT2) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000 (center and green). Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65513-1-PBS in a different storage buffer formulation.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Product Information
65513-1-PBS targets CD5 in FC applications and shows reactivity with human samples.
Store at -80°C. Stable for one year after shipment.
Background Information
CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65513-1-RR (same clone as 65513-1-PBS)
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-RR, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65513-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65513-1-RR (same clone as 65513-1-PBS)
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-RR, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65513-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65513-1-PBS
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65513-1-PBS
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000 (left and red), or stained with 0.2 ug Anti-Human CD5 Mouse Monoclonal Antibody (65152-1-Ig, Clone:UCHT2) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000 (center and green). Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65513-1-RR (same clone as 65513-1-PBS)
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-RR, Clone:UCHT2) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000 (left and red), or stained with 0.2 ug Anti-Human CD5 Mouse Monoclonal Antibody (65152-1-Ig, Clone:UCHT2) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) at dilution 1:1000 (center and green). Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65513-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD5 Recombinant antibody
Catalog Number
65513-1-PBS
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
