CD5 Polyclonal antibody

CD5 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17227-1-AP

Synonyms

T-cell surface glycoprotein CD5, T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1

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Validation Data Gallery

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  WB analysis of Jurkat using 17227-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17227-1-AP (CD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17227-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17227-1-AP (CD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 17227-1-AP

  IP result of anti-CD5 (IP:17227-1-AP, 4ug; Detection:17227-1-AP 1:500) with mouse liver tissue lysate 6800ug.

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  IHC staining of human tonsillitis using 17227-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17227-1-AP (CD5 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17227-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17227-1-AP (CD5 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 17227-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD5 antibody (17227-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 17227-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD5 antibody (17227-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

17227-1-AP targets CD5 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD5 fusion protein Ag11065
• Full Name: CD5 molecule
• Calculated Molecular Weight: 495 aa, 55 kDa
• Observed Molecular Weight: 55 kDa, 67 kDa
• GenBank Accession Number: BC027901
• Gene Symbol: CD5
• Gene ID (NCBI): 921
• RRID: AB_2075581
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

Publications

Species	Application	Title
mouse	IHC	FASEB J; A self-organized actomyosin drives multiple intercellular junction disruption and directly promotes neutrophil recruitment in lipopolysaccharide-induced acute lung injury.; Authors - Bing Chen
human	IHC	Front Oncol; Whole-Exome Sequencing Reveals New Potential Mutations Genes for Primary Mucosa-Associated Lymphoid Tissue Lymphoma Arising From the Kidney.; Authors - Shuang Wen
human	WB	Hematol Oncol; Nuclear αvβ3 integrin expression, post translational modifications and regulation in hematological malignancies.; Authors - Avivit Weisz