CoraLite® Plus 488 Anti-Mouse CD49b (DX5)

CD49b Monoclonal Antibody for FC

Host / Isotype

Rat / IgM, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

DX5

Cat no : CL488-65065

Synonyms

CD49B, DX5, integrin alpha 2, Itga2



Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65065 targets CD49b in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgM, kappa
Class Monoclonal
Type Antibody
Immunogen NK cells isolated from C57BL/6 mice
Full Name integrin alpha 2
GenBank Accession NumberBC065139
Gene Symbol CD49b
Gene ID (NCBI) 16398
RRIDAB_2883158
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and CLG gene expression, force generation and organization of newly synthesized extracellular matrix.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD49b antibody CL488-65065Download protocol
Standard Protocols
Click here to view our Standard Protocols