Anti-Human CD49b (AK7)

CD49b Monoclonal Antibody for FC, ELISA

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

AK7

Cat no : 65252-1-Ig

Synonyms

BR, CD49B, Collagen receptor, GPIa, Integrin alpha 2, ITGA2, VLA 2, VLA 2 subunit alpha, VLAA2

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using 65252-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD49b (65252-1-Ig, Clone:AK7) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65252-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD49b (65252-1-Ig, Clone:AK7) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Monocytes were gated.

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65252-1-Ig targets CD49b in FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human platelets
• Full Name: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• Calculated Molecular Weight: 126 kDa
• GenBank Accession Number: NM_002203
• Gene Symbol: Integrin alpha 2
• Gene ID (NCBI): 3673
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD49b, also known as alpha2 integrin, is a type I transmembrane glycoprotein which forms an adhesion molecule VLA-2 with beta1 integrin. CD49b is a receptor for many (extracellular) matrix and non-matrix molecules (PMID: 23847623). CD49b is expressed on numerous cell types including platelets, activated T cells, B cells, and monocytes. CD49b activation skews NK cell effector function to a phenotype that exhibits elevated IFN-γ production with a concomitant inhibition of motility and cytolytic function (PMID: 32971468, 32762681). The Anti-Human CD49b antibody, clone AK7, is derived from Human platelets.