Anti-Human CD48 Rabbit Recombinant Antibody, PBS Only

CD48 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241389F8

Cat no : 98170-1-PBS

Synonyms

CD48 molecule, CD48 antigen, B-lymphocyte activation marker BLAST-1, BLAST1, BLAST

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocytes using 98170-1-RR (same clone as 98170-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD48 Rabbit Recombinant Antibody (98170-1-RR, Clone:241389F8) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98170-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98170-1-RR (same clone as 98170-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD48 Rabbit Recombinant Antibody (98170-1-RR, Clone:241389F8) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98170-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98170-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98170-1-RR against Human CD48 were performed. The affinity constant is 0.383 nM.

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Product Information

The immunogen of 98170-1-PBS is CD48 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD48 molecule
• Calculated Molecular Weight: 43 kDa
• GenBank Accession Number: BC016182
• Gene Symbol: CD48
• Gene ID (NCBI): 962
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.