CD48 Polyclonal antibody
CD48 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 27519-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Raji cells, Daudi cells, Ramos cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
27519-1-AP targets CD48 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD48 fusion protein Ag26536 |
Full Name | CD48 molecule |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 40-45 kDa |
GenBank Accession Number | BC016182 |
Gene Symbol | CD48 |
Gene ID (NCBI) | 962 |
RRID | AB_2880897 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.
Protocols
Product Specific Protocols | |
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WB protocol for CD48 antibody 27519-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |