CD48 Polyclonal antibody

CD48 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27519-1-AP

Synonyms

hCD48, CD48 molecule, CD48 antigen, B-lymphocyte activation marker BLAST-1, BLAST1

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Validation Data Gallery

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  WB analysis of Raji using 27519-1-AP

  Raji cells and Ramos cells were subjected to SDS PAGE followed by western blot with 27519-1-AP (CD48 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 27519-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 27519-1-AP (CD48 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Raji cells, Daudi cells, Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27519-1-AP targets CD48 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD48 fusion protein Ag26536
• Full Name: CD48 molecule
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC016182
• Gene Symbol: CD48
• Gene ID (NCBI): 962
• RRID: AB_2880897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.