CD46 Polyclonal antibody, PBS Only

CD46 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28936-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 28936-1-AP (same clone as 28936-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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  WB analysis using 28936-1-AP (same clone as 28936-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 28936-1-AP (same clone as 28936-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 28936-1-AP (CD46 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 28936-1-AP (same clone as 28936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28936-1-AP (CD46 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 28936-1-AP (same clone as 28936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28936-1-AP (CD46 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28936-1-PBS in a different storage buffer formulation.

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Product Information

28936-1-PBS targets CD46 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD46 fusion protein Ag30376
• Full Name: CD46 molecule, complement regulatory protein
• Calculated Molecular Weight: 384 aa, 43 kDa
• Observed Molecular Weight: 50-70 kDa
• GenBank Accession Number: BC030594
• Gene Symbol: CD46
• Gene ID (NCBI): 4179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).