CD46 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD46 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 12494-1-AP

Synonyms

TRA2.10, TLX, MIC10, Membrane cofactor protein, MCP

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Validation Data Gallery

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  WB analysis using 12494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12494-1-AP (CD46 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 12494-1-AP

  IP result of anti-CD46 (IP:12494-1-AP, 4ug; Detection:12494-1-AP 1:1000) with A549 cells lysate 1800ug.

• 

  IHC staining of human ovary tumor using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12494-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CD46 antibody (12494-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, Jurkat cells, MOLT-4 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue, human colon cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• FC: See 1 publications below

Product Information

12494-1-AP targets CD46 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD46 fusion protein Ag3131
• Full Name: CD46 molecule, complement regulatory protein
• Calculated Molecular Weight: 384 aa, 43 kDa
• Observed Molecular Weight: 50-56 kDa
• GenBank Accession Number: BC030594
• Gene Symbol: CD46
• Gene ID (NCBI): 4179
• RRID: AB_2260042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).

Publications

Species	Application	Title
human	WB, FC	Mol Immunol; Functional characterization of two novel non-synonymous alterations in CD46 and a Q950H change in factor H found in atypical hemolytic uremic syndrome patients.; Authors - Frida C Mohlin
human	WB	Oncol Rep; Bioinformatic analysis of the membrane cofactor protein CD46 and microRNA expression in hepatocellular carcinoma.; Authors - Zejun Lu ; KD Validated
mouse	WB	Clin Exp Immunol; Mitigating placental injuries through up-regulating DAF in experimental APS mice:new mechanism of progesterone.; Authors - Yingchun Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrea (Verified Customer) (04-12-2022)

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa cells

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