Anti-Rat CD45RA (OX-33) Mouse IgG2a Recombinant Antibody, PBS Only

CD45RA Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

rat

Applications

FC

Conjugate

Unconjugated

CloneNo.

OX-33

Cat no : 65578-1-PBS

Synonyms

Lca, Ptprc, CD45, RT7, OX-33

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Validation Data Gallery

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  FC experiment of rat splenocytes using 65578-1-MR (same clone as 65578-1-PBS)

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone: OX-33) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and FITC anti-Mouse IgG2a Antibody. Cells were then stained with APC Anti-Rat CD4. Cells were not fixed. This data was developed using the same antibody clone with 65578-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat splenocytes using 65578-1-MR (same clone as 65578-1-PBS)

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone:OX-33) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed. This data was developed using the same antibody clone with 65578-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat splenocytes using 65578-1-MR (same clone as 65578-1-PBS)

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone: OX-33) and FITC anti-Mouse IgG2a Antibody (left), or 0.25 ug Anti-Rat CD45RA (65234-1-Ig, Clone: OX-33) and FITC anti-Mouse IgG1 Antibody (right). Cells were then stained with APC Anti-Rat CD4. Cells were not fixed. This data was developed using the same antibody clone with 65578-1-PBS in a different storage buffer formulation.

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Product Information

65578-1-PBS targets CD45RA in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Leukocyte common antigen purified from rat splenocytes
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 143 kDa
• GenBank Accession Number: XM_006249910
• Gene Symbol: CD45
• Gene ID (NCBI): 24699
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signaling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).