Anti-Human CD45RA (F8-11-13)

CD45RA Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

F8-11-13

Cat no : 65226-1-Ig

Synonyms

CD45, B220, GP180, F8-11-13, EC:3.1.3.48

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using 65226-1-Ig

  1X10^6 human PBMCs were surface co-stained with PE Anti-human CD3, FITC Anti-human CD45RO, and 0.2 ug Anti-Human CD45RA (65226-1-Ig, Clone: F8-11-13) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65226-1-Ig

  1X10^6 human PBMCs were surface co-stained with PE Anti-human CD3 and 0.2 ug Anti-Human CD45RA (65226-1-Ig, Clone: F8-11-13) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65226-1-Ig targets CD45RA in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human T lymphocytes
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 1304 aa, 147 kDa
• GenBank Accession Number: BC014239
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).