Anti-Human CD45RA (HI100), PBS Only

• 

  FC experiment of human PBMCs using 65108-1-Ig (same clone as 65108-1-PBS)

  1X10^6 human PBMCs were surface co-stained with CL647 Anti-Human CD45RO and 0.2 ug Anti-Human CD45RA (65108-1-Ig, Clone:HI100) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65108-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65108-1-Ig (same clone as 65108-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45RA (65108-1-Ig, Clone:HI100) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or unstained. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65108-1-PBS in a different storage buffer formulation.

CD45RA Monoclonal Antibody for Indirect ELISA

Cat No. 65108-1-PBS

Clone No.HI100

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

Indirect ELISA

Synonyms

CD45, B220, HI100, GP180, EC:3.1.3.48

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Product Information

65108-1-PBS targets CD45RA in Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• ENSEMBL Gene ID: ENSG00000081237
• RRID: AB_2881472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P08575
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).