Filter: AllWBIHCIF/ICCAffinity

WB analysis using 83396-5-RR (same clone as 83396-5-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83396-5-RR (CD45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 83396-5-RR (same clone as 83396-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 83396-5-RR (same clone as 83396-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.
IHC staining of mouse spleen using 83396-5-RR (same clone as 83396-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.
IHC staining of mouse spleen using 83396-5-RR (same clone as 83396-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.
IF Staining of RAW 264.7 using 83396-5-RR (same clone as 83396-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD45/PTPRC antibody (83396-5-RR, Clone: 240356G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.
IF Staining of RAW 264.7 using 83396-5-RR (same clone as 83396-5-PBS)
Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD45/PTPRC antibody (83396-5-RR, Clone: 240356G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83396-5-RR
Biolayer interferometry (BLl) kinetic assays of 83396-5-RR against Mouse CD45 were performed. The affinity constant is 0.415 nM.

Mouse CD45 Recombinant antibody, PBS Only

CD45 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 83396-5-PBS

Clone No.240356G10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CD45/PTPRC, Ptprc, L-CA, LCA, EC:3.1.3.48

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

83396-5-PBS targets CD45 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Recombinant protein Predict reactive species
Full Name	protein tyrosine phosphatase, receptor type, C
Calculated Molecular Weight	145 kDa
Observed Molecular Weight	190 kDa
GenBank Accession Number	NM_001111316.2
Gene Symbol	CD45
Gene ID (NCBI)	19264
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	P06800-4
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).