CD45 Recombinant antibody, PBS Only

CD45 Recombinant Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat, Mouse

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6O19

Cat no : 80297-1-PBS

Synonyms

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Validation Data Gallery

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  WB analysis using 80297-1-RR (same clone as 80297-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80297-1-RR (CD45 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 80297-1-RR (same clone as 80297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 80297-1-RR (same clone as 80297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

  Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 80297-1-RR (same clone as 80297-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (80297-1-RR, Clone: 6O19 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 80297-1-RR (same clone as 80297-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD45 antibody (80297-1-RR, Clone: 6O19 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 80297-1-RR (same clone as 80297-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD45 antibody (80297-1-RR, Clone: 6O19 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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  FC experiment of Raji using 80297-1-RR (same clone as 80297-1-PBS)

  1X10^6 Raji cells were intracellularly stained with 0.15 ug Anti-Human CD45 (80297-1-RR, Clone: 6O19) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.15 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80297-1-PBS targets CD45 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD45 fusion protein Ag13712
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 1304 aa, 147 kDa
• Observed Molecular Weight: 220-240 kDa
• GenBank Accession Number: BC014239
• Gene Symbol: PTPRC
• Gene ID (NCBI): 5788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.