CD45 Polyclonal antibody

CD45 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31243-1-AP

Synonyms

B220, Cd45, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200

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Validation Data Gallery

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  WB analysis using 31243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31243-1-AP (CD45/PTPRC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: EL-4 cells, J774A.1 cells, RAW 264.7 cells, mouse spleen tissue, mouse thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31243-1-AP targets CD45 in WB, ELISA applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 145 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: NM_001111316.2
• Gene Symbol: CD45R
• Gene ID (NCBI): 19264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).