Mouse CD45.1 Recombinant antibody, PBS Only

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  WB analysis using 84325-2-RR (same clone as 84325-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84325-2-RR (Mouse CD45.1-ly5a-Domain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84325-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84325-2-RR (same clone as 84325-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84325-2-RR (CD45.1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84325-2-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 84325-2-RR (same clone as 84325-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD45.1 antibody (84325-2-RR, Clone: 240921A2) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat-mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84325-2-PBS in a different storage buffer formulation.

CD45.1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 84325-2-PBS

Clone No.240921A2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Cd45, Ptprc, L-CA, LCA, EC:3.1.3.48

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Product Information

84325-2-PBS targets CD45.1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein tyrosine phosphatase, receptor type, C
• Observed Molecular Weight: 200 kDa
• Gene Symbol: CD45
• Gene ID (NCBI): 19264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).