FITC Plus Anti-Human CD44 (F10-44-2)

CD44 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human

Applications

IF, FC

Conjugate

FITC Plus Fluorescent Dye

Publications

3

CloneNo.

F10-44-2

Cat no : FITC-65063

Synonyms

Epican, ECMR-III, ECMR III, CSPG8, CDW44

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Validation Data Gallery

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  FC experiment of human PBMCs using FITC-65063

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD3, and 5 ul FITC Plus Anti-Human CD44 (F10-44-2) (FITC-65063, Clone: F10-44-2) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65063

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD44 (F10-44-2) (FITC-65063, Clone: F10-44-2) (red) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• FC: See 2 publications below

Product Information

FITC-65063 targets CD44 in IF, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Purified T cells from human lymph nodes
• Full Name: CD44 molecule (Indian blood group)
• Calculated Molecular Weight: 742 aa, 82 kDa
• GenBank Accession Number: BC004372
• Gene Symbol: CD44
• Gene ID (NCBI): 960
• RRID: AB_2883753
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Publications

Species	Application	Title
human	FC	ACS Nano; Inhibition Effect of Ti3C2Tx MXene on Ice Crystals Combined with Laser-Mediated Heating Facilitates High-Performance Cryopreservation.; Authors - Yuan Cao
human	IF	Free Radic Biol Med; Retinal cell-targeted liposomal ginsenoside Rg3 attenuates retinal ischemia-reperfusion injury via alleviating oxidative stress and promoting microglia/macrophage M2 polarization; Authors - Yanmei Huang
human	FC	Open Life Sci; Experimental study on the optimization of ANM33 release in foam cells; Authors - Chen Yuan