FITC Plus Anti-Human CD44 (F10-44-2)

CD44 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human and More (1)

Applications

IF, FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

F10-44-2

Cat no : FITC-65063

Synonyms

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I



Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

FITC-65063 targets CD44 in IF, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Purified T cells from human lymph nodes
Full Name CD44 molecule (Indian blood group)
Calculated molecular weight 742 aa, 82 kDa
GenBank accession numberBC004372
Gene symbol CD44
Gene ID (NCBI) 960
RRIDAB_2883753
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Protocols

Product Specific Protocols
FC protocol for FITC Plus CD44 antibody FITC-65063Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

ACS Nano

Inhibition Effect of Ti3C2Tx MXene on Ice Crystals Combined with Laser-Mediated Heating Facilitates High-Performance Cryopreservation.

Authors - Yuan Cao
humanIF

Free Radic Biol Med

Retinal cell-targeted liposomal ginsenoside Rg3 attenuates retinal ischemia-reperfusion injury via alleviating oxidative stress and promoting microglia/macrophage M2 polarization

Authors - Yanmei Huang
humanFC

Open Life Sci

Experimental study on the optimization of ANM33 release in foam cells

Authors - Chen Yuan