CD44 Monoclonal antibody, PBS Only

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  WB analysis using 60224-1-Ig (same clone as 60224-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  WB analysis using 60224-1-Ig (same clone as 60224-1-PBS)

  HeLa (CD44+), A549 (CD44+), MDA-MB-231 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa cells using 60224-1-Ig (same clone as 60224-1-PBS)

  WB result of CD44 antibody (60224-1-Ig, 1:4000) with si-Control and si-CD44 transfected HeLa cells. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  WB analysis of human spleen using 60224-1-Ig (same clone as 60224-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60224-1-Ig (same clone as 60224-1-PBS)

  IP result of anti-CD44 (IP:60224-1-Ig, 4ug; Detection:60224-1-Ig 1:3000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60224-1-Ig (CD44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60224-1-Ig (CD44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunofluorescent analysis of un-fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CD44 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 60224-1-PBS

Clone No.6F4H2

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen

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Product Information

60224-1-PBS targets CD44 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD44 fusion protein Ag7633
• Full Name: CD44 molecule (Indian blood group)
• Calculated Molecular Weight: 742 aa, 82 kDa
• Observed Molecular Weight: 80-95 kDa
• GenBank Accession Number: BC004372
• Gene Symbol: CD44
• Gene ID (NCBI): 960
• ENSEMBL Gene ID: ENSG00000026508
• RRID: AB_11042767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16070
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.