CD41/Integrin alpha 2b Polyclonal antibody, PBS Only

CD41/Integrin alpha 2b Polyclonal Antibody for IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18308-1-PBS

Synonyms

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Validation Data Gallery

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  IHC staining of mouse spleen using 18308-1-AP (same clone as 18308-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 18308-1-AP (CD41/Integrin alpha 2b antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18308-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 18308-1-AP (same clone as 18308-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 18308-1-AP (CD41/Integrin alpha 2b antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18308-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 18308-1-AP (same clone as 18308-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 18308-1-AP (CD41/Integrin Alpha 2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18308-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 18308-1-AP (same clone as 18308-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 18308-1-AP (CD41/Integrin Alpha 2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18308-1-PBS in a different storage buffer formulation.

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Product Information

18308-1-PBS targets CD41/Integrin alpha 2b in IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
• Calculated Molecular Weight: 113 kDa
• GenBank Accession Number: BC126442
• Gene Symbol: CD41a
• Gene ID (NCBI): 3674
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light (25 kDa) and heavy (125 kDa) chains which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrin alpha-IIb/beta-3 is crucial for platelet aggregation through binding of soluble fibrinogen.