Mouse CD40 Recombinant antibody, PBS Only

CD40 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240874B2

Cat no : 83972-5-PBS

Synonyms

Tnfrsf5, CD40 antigen, Bp50, 240874B2

---

Validation Data Gallery

• 

  WB analysis using 83972-5-RR (same clone as 83972-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83972-5-RR (CD40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83972-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 83972-5-RR (same clone as 83972-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83972-5-RR (CD40 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83972-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 83972-5-RR (same clone as 83972-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83972-5-RR (CD40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83972-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83972-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83972-5-RR against Mouse CD40 were performed. The affinity constant is 4.50 nM.

---

Product Information

The immunogen of 83972-5-PBS is CD40 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD40 antigen
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: NM_011611.2
• Gene Symbol: Cd40
• Gene ID (NCBI): 21939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.