CD40 Monoclonal antibody, PBS Only

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  WB analysis of Raji using 66965-1-Ig (same clone as 66965-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66965-1-Ig (CD40 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 66965-1-Ig (same clone as 66965-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66965-1-Ig (CD40 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66965-1-Ig (same clone as 66965-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human follicular lymphoma tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66965-1-Ig (same clone as 66965-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human follicular lymphoma tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

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  IF Staining of Ramos using 66965-1-Ig (same clone as 66965-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 66965-1-Ig (same clone as 66965-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

CD40 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 66965-1-PBS

Clone No.2A8G5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

p50, CD40L receptor, Bp50, B-cell surface antigen CD40, B cell surface antigen CD40

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Product Information

66965-1-PBS targets CD40 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD40 fusion protein Ag28040
• Full Name: CD40 molecule, TNF receptor superfamily member 5
• Calculated Molecular Weight: 277 aa, 31 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC012419
• Gene Symbol: CD40
• Gene ID (NCBI): 958
• ENSEMBL Gene ID: ENSG00000101017
• RRID: AB_2882288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P25942
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.