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CD4 Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG1, kappa

Detection: Mouse / IgG1

Reactivity

Capture: human

Detection: Human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 15.6-1000 pg/mL

Cat No : MP50077-1

Print datasheet

Synonyms

CD4, CD4 molecule, CD4mut

Validation Data Gallery

  • Sandwich ELISA standard curve of MP50077-1, CD4 Monoclonal Matched Antibody Pair - PBS only. 65147-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0333. 68688-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

    Sandwich ELISA standard curve of MP50077-1

    Sandwich ELISA standard curve of MP50077-1, CD4 Monoclonal Matched Antibody Pair - PBS only. 65147-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0333. 68688-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP50077-1 targets CD4 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 15.6-1000 pg/mL (Sandwich ELISA);

Capture antibody: Anti-Human CD4 (SK3), PBS Only 65147-1-PBS (SK3). 100 μg. Concentration 1 mgl/ml.

Detection antibody: CD4 Monoclonal antibody, PBS Only (Detector) 68688-1-PBS (1E11B4). 100 μg. Concentration 1 mgl/ml.

Alternative CD4 matched antibody pairs: MP00503-1, MP00503-2, MP00503-3

Matched pair protein standard: Eg0333

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 65147-1-PBS
Tested Reactivity human
Immunogen N/A
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Affinity
KD=8.82 x 10-11M
KOff=2.00 x 10-5M
KOn=2.26 x 105M
Full Name CD4 molecule
Synonyms T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, SK3, CD4mut, CD4 molecule
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 68688-1-PBS
Tested Reactivity Human
Immunogen Recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.39 x 10-10M
KOff=4.36 x 10-6M
KOn=3.13 x 104M
Full Name CD4 molecule
Synonyms CD4, CD4 molecule, CD4mut
GenBank accession numberBC025782
Gene symbol CD4
UniprotP01730
Gene ID (NCBI) 920
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.