Biotin Anti-Human CD4 (OKT4) Rabbit IgG Recombinant Antibody

CD4 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Biotin

CloneNo.

OKT4

Cat no : Biotin-65505

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, OKT4, CD4mut, CD4 molecule

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Validation Data Gallery

• 

  FC experiment of human PBMCs using Biotin-65505

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD4 Rabbit IgG Recombinant Antibody (Biotin-65505, Clone: OKT4) and CoraLite®488-conjugated streptavidin, or unstained. Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using Biotin-65505

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD4 Rabbit IgG Recombinant Antibody (Biotin-65505, Clone: OKT4) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65505 targets CD4 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Human peripheral T cells
• Full Name: CD4 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).