CD4 Monoclonal antibody, PBS Only

CD4 Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H4A2

Cat no : 67786-1-PBS

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 2H4A2

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Validation Data Gallery

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  IHC staining of human tonsillitis using 67786-1-Ig (same clone as 67786-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67786-1-Ig (same clone as 67786-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

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  IHC staining of human thymus using 67786-1-Ig (same clone as 67786-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67786-1-Ig (same clone as 67786-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67786-1-Ig (same clone as 67786-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thymus using 67786-1-Ig (same clone as 67786-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 67786-1-Ig (same clone as 67786-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 67786-1-Ig (same clone as 67786-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 67786-1-Ig (same clone as 67786-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD4 antibody (67786-1-Ig, Clone: 2H4A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

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Product Information

67786-1-PBS targets CD4 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD4 fusion protein Ag30870
• Full Name: CD4 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).