Anti-Human CD4 (OKT4) Mouse IgG2a Recombinant Antibody, PBS Only

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  FC experiment of human PBMCs using 65603-1-MR (same clone as 65603-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Mouse IgG2a Recombinant Antibody (65603-1-MR, Clone: OKT4) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65603-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65603-1-MR (same clone as 65603-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Mouse IgG2a Recombinant Antibody (65603-1-MR, Clone: OKT4) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65603-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65603-1-MR (same clone as 65603-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Mouse IgG2a Recombinant Antibody (65603-1-MR, Clone: OKT4) (left) or 0.25 ug Anti-Human CD4 (65134-1-Ig, Clone: OKT4) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65603-1-PBS in a different storage buffer formulation.

CD4 {ptg:Brand} Recombinant Antibody for FC

Cat No. 65603-1-PBS

Clone No.OKT4

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, OKT4, CD4mut, CD4 molecule

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Product Information

65603-1-PBS targets CD4 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD4 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• ENSEMBL Gene ID: ENSG00000010610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).