Anti-Human CD4 (OKT4) Rabbit Recombinant Antibody, PBS only

CD4 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

OKT4

Cat no : 65505-1-PBS

Synonyms

CD4, CD4 molecule, CD4mut

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Validation Data Gallery

• 

  FC experiment of human PBMCs using 65505-1-PBS

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-PBS, Clone:OKT4) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65505-1-PBS

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-PBS, Clone:OKT4) or Rabbit IgG control PolyAb, and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65505-1-PBS

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD4 Rabbit Recombinant Antibody (65505-1-PBS, Clone:OKT4) and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (left) or 0.1 ug Anti-Human CD4 Mouse Monoclonal Antibody (Clone:OKT4) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and right). Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65505-1-PBS targets CD4 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Human peripheral T cells
• Full Name: CD4 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).