FC experiment of mouse splenocytes using 65199-1-Ig
1X10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD4 (65199-1-Ig, Clone:RM4-4) and FITC anti-rat IgG2b Antibody at dilution 1:100 (red), or 0.25 ug Control Antibody. Cells were not fixed.
1X10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD4 (65199-1-Ig, Clone:RM4-4) and FITC anti-rat IgG2b Antibody at dilution 1:100 (red), or 0.25 ug Control Antibody. Cells were not fixed.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
65199-1-Ig targets CD4 in FC applications and shows reactivity with Mouse samples.
Store at 2-8°C. Stable for one year after shipment.
Background Information
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
FC experiment of mouse splenocytes using 65199-1-Ig
1X10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD4 (65199-1-Ig, Clone:RM4-4) and FITC anti-rat IgG2b Antibody at dilution 1:100 (red), or 0.25 ug Control Antibody. Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD4 Monoclonal antibody
Catalog Number
65199-1-Ig
Citations
-
Dilutions
Applications
FC
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
