Anti-Rat CD4 (OX-35)

CD4 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG2a kappa

Reactivity

Rat

Applications

FC

Conjugate

Unconjugated

CloneNo.

OX-35

Cat no : 65179-1-Ig

Synonyms

CD4, CD4 molecule, p55, W3/25

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Validation Data Gallery

• 

  FC experiment of wistar rat splenocytes using 65179-1-Ig

  1X10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD4 (65179-1-Ig, Clone:OX-35) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.25 ug Mouse IgG2a isotype control and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: rat splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65179-1-Ig targets CD4 in FC applications and shows reactivity with Rat samples.

• Tested Reactivity: Rat
• Host / Isotype: Mouse / Mouse IgG2a kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: MLR generated rat T helper lymphocytes
• Full Name: Cd4 molecule
• Gene Symbol: Cd4
• Gene ID (NCBI): 24932
• RRID: AB_2918454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).