CD3EAP Polyclonal antibody

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  WB analysis using 31162-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31162-1-AP (CD3EAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 31162-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CD3EAP antibody (31162-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CD3EAP Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31162-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

A34.5, Antisense to ERCC 1 protein, ASE 1, ASE1, CAST, CD3 epsilon associated protein, CD3E associated protein, CD3EAP, PAF49

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Tested Applications

• Positive WB detected in: A431 cells, A549 cells, HeLa cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31162-1-AP targets CD3EAP in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD3EAP fusion protein Ag35074
• Full Name: CD3e molecule, epsilon associated protein
• Calculated Molecular Weight: 510 aa, 55 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC038992
• Gene Symbol: CD3EAP
• Gene ID (NCBI): 10849
• RRID: AB_3669876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O15446
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 and as CD3ɛ-associated signal transducer (CAST), CD3EAP is a 55 kDa nucleolar autoantigen that has an apparent molecular mass of 90 kDa (PMID: 11199923). CD3EAP/hPAF49 contains a single tyrosine residue at position 82 (Tyr82), which is phosphorylated upon stimulation of the T-cell receptor. Western blot analysis detected CD3EAP at an apparent molecular mass of ~80-90 kDa.