CoraLite® Plus 647 Anti-Mouse CD38 (90)

CD38 Monoclonal Antibody for IF, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

90

Cat no : CL647-65059

Synonyms

Cd38, CD38 antigen, Cd38 rs1

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Validation Data Gallery

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  IF Staining of mouse splenocytes using CL647-65059

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD38 (CL647-65059, Clone: 90) at dilution of 1:400.

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  FC experiment of mouse splenocytes using CL647-65059

  1X10^6 mouse splenocytes were surface stained with 0.2 ug CoraLite® Plus 647 Anti-Mouse CD38 (CL647-65059, Clone:90) or 0.2 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3), and 0.2 ug FITC Plus Anti-Mouse CD3ε (145-2C11) (FITC-65060, Clone: 145-2C11). Cells were not fixed.

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Tested Applications

• Positive IF detected in: mouse splenocytes
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65059 targets CD38 in IF, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse bone marrow pre-B cells
• Full Name: CD38 antigen
• GenBank Accession Number: BC046312
• Gene Symbol: Cd38
• Gene ID (NCBI): 12494
• RRID: AB_2883660
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).