CoraLite® Plus 488 Anti-Human CD38 (HB7)

CD38 Monoclonal Antibody for IF, FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

HB7

Cat no : CL488-65107

Synonyms

ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1, T10

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Validation Data Gallery

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  IF Staining of human peripheral blood mononuclear cells using CL488-65107

  Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using CoraLite® Plus 488-conjugated Anti-Human CD38 (CL488-65107, Clone:HB7) at dilution of 1:400.

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  FC experiment of human peripheral blood lymphocytes using CL488-65107

  100 ul human peripheral blood were surface stained 5.00 ul/test CoraLite® Plus 488-conjugated Anti-Human CD38 (CL488-65107, Clone:HB7) (red) or isotype control antibody (blue). Lymphocytes were gated for analysis. Cells were not fixed.

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Tested Applications

• Positive IF/ICC detected in: human peripheral blood mononuclear cells
• Positive FC detected in: human peripheral blood lymphocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65107 targets CD38 in IF, FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human BJAB B cell line
• Full Name: CD38 molecule
• Calculated Molecular Weight: 300 aa, 34 kDa
• GenBank Accession Number: BC007964
• Gene Symbol: CD38
• Gene ID (NCBI): 952
• RRID: AB_2883181
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).