CoraLite® Plus 405 Anti-Human CD38 (HIT2)

CD38 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

HIT2

Cat no : CL405-65111

Synonyms

ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1, T10

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Validation Data Gallery

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  FC experiment of human PBMCs using CL405-65111

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD38 (CL405-65111, Clone:HIT2), or CL405 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65111 targets CD38 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD38 molecule
• Calculated Molecular Weight: 300 aa, 34 kDa
• GenBank Accession Number: BC007964
• Gene Symbol: CD38
• Gene ID (NCBI): 952
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).