CD38 Monoclonal antibody, PBS Only

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  WB analysis using 60006-1-Ig (same clone as 60006-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60006-1-Ig (CD38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60006-1-Ig (same clone as 60006-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60006-1-Ig (CD38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 60006-1-Ig (same clone as 60006-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 60006-1-Ig (same clone as 60006-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Raji using 60006-1-Ig (same clone as 60006-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60006-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CD38 Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA

Cat No. 60006-1-PBS

Clone No.3C6G4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, IF-P, ELISA

Synonyms

ADPRC 1, ADP ribosyl cyclase 1, 3C6G4, 2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase

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Product Information

60006-1-PBS targets CD38 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD38 fusion protein Ag0487
• Full Name: CD38 molecule
• Calculated Molecular Weight: 300 aa, 34 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC007964
• Gene Symbol: CD38
• Gene ID (NCBI): 952
• ENSEMBL Gene ID: ENSG00000004468
• RRID: AB_716994
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P28907
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).