CD37 Recombinant antibody

• 

  WB analysis using 84509-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84509-2-RR (CD37 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 84509-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84509-2-RR (CD37 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84509-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84509-2-RR (CD37 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Daudi using 84509-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CD37 antibody (84509-2-RR, Clone: 242011D6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD37 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 84509-2-RR

Clone No.242011D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Tspan 26, Tetraspanin 26, Leukocyte antigen CD37, GP52 40, CD37 molecule

---

Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Daudi cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84509-2-RR targets CD37 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FusionProtein
• Full Name: CD37 molecule
• Calculated Molecular Weight: 32kDa
• Observed Molecular Weight: 40-52 kDa
• GenBank Accession Number: NM_001774.3
• Gene Symbol: CD37
• Gene ID (NCBI): 951
• RRID: AB_3672018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11049-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).