Tested Applications
Positive WB detected in | Daudi cells, Raji cells, Ramos cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Daudi cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
84509-2-RR targets CD37 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | FusionProtein Predict reactive species |
Full Name | CD37 molecule |
Calculated Molecular Weight | 32kDa |
Observed Molecular Weight | 40-52 kDa |
GenBank Accession Number | NM_001774.3 |
Gene Symbol | CD37 |
Gene ID (NCBI) | 951 |
RRID | AB_3672018 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P11049-1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).
Protocols
Product Specific Protocols | |
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WB protocol for CD37 antibody 84509-2-RR | Download protocol |
IHC protocol for CD37 antibody 84509-2-RR | Download protocol |
IF protocol for CD37 antibody 84509-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |