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CD36 Polyclonal antibody
CD36 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, hamster and More (2)
Applications
WB, IHC, FC, ELISA
Conjugate
Unconjugated
Cat no : 18836-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Human peripheral blood platelets, mouse spleen tissue, THP-1 cells, mouse liver tissue, HepG2 cells, RAW 264.7 cells |
| Positive IHC detected in | human spleen tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 79 publications below |
| IHC | See 13 publications below |
| IF | See 9 publications below |
Product Information
18836-1-AP targets CD36 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, hamster samples.
| Tested Reactivity | human, mouse, hamster |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | CD36 molecule (thrombospondin receptor) |
| Calculated molecular weight | 472 aa, 53 kDa |
| Observed molecular weight | 88 kDa |
| GenBank accession number | BC008406 |
| Gene symbol | CD36 |
| Gene ID (NCBI) | 948 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Function
CD36, also known as platelet glycoprotein 4, is an integral membrane glycoprotein that acts as a scavenger receptor. CD36 can bind to multiple ligands, including thrombospondin-1, collagen, oxidized phospholipids, oxidized low-density lipoprotein, and long-chain fatty acids. CD36 can also bind to erythrocytes parasitized by Plasmodium falciparum and apoptotic cells. CD36 mediates different biological processes, acting as a signaling hub in angiogenesis, inflammatory response, and fatty acid metabolism.
Tissue specificity
CD36 is present on the surface of various cells types, such as adipocytes, monocytes, macrophages, platelets, microvascular endothelial cells, dendritic cells, and hematopoietic precursors of red cells.
Involvement in disease
Mutations in CD36 can give rise to platelet glycoprotein IV deficiency, a type of macrothrombocytopenia.
Polymorphisms in CD36 can increase susceptibility to malaria.
AAGIC haplotype at the CD36 locus increases the risk of coronary heart disease.
Disruption of CD36-dependent pathways and certain SNPs in the CD36 gene are attributed to impaired fatty acid metabolism, glucose intolerance, Alzheimer's disease, atherosclerosis, arterial hypertension, diabetes, and cardiomyopathy.
Isoforms
Apart from the full-length protein (isoform 1), one additional shorter isoform has been reported (PMID: 7509795). Other alternative isoforms have also been detected on the mRNA level (PMID: 17673938).
Post-translational modifications
The extracellular domain of CD36 is extensively glycosylated. Glycosylation is needed for the transport of CD36 to the plasma membrane, as well as mediating recognition and binding to ligands. Cytoplasmic tails of transmembrane domains can be phosphorylated and play a role in signal transduction. Intracellular domains can be additionally acetylated, ubiquitinated, and palmitoylated (PMID: 28919632).
Cellular localization
CD36 is present on the cell surface.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD36 antibody 18836-1-AP | Download protocol |
| IHC protocol for CD36 antibody 18836-1-AP | Download protocol |
| FC protocol for CD36 antibody 18836-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogenesis CD36 plays a critical role in proliferation, migration and tamoxifen-inhibited growth of ER-positive breast cancer cells. | ||
J Obstet Gynaecol Res Eutopic endometrium from patients with endometriosis modulates the expression of CD36 and SIRP-α in peritoneal macrophages. | ||
Biochem Biophys Res Commun ALDH2 deficiency inhibits Ox-LDL induced foam cell formation via suppressing CD36 expression. | ||
J Anim Sci Antimicrobial peptide KR-32 alleviates Escherichia coli K88-induced fatty acid malabsorption by improving expression of FATP4. | ||
Physiol Rep Adropin reduces blood glucose levels in mice by limiting hepatic glucose production. | ||
Sci Rep An in vitro model of foam cell formation induced by a stretchable microfluidic device. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Marsilius (Verified Customer) (02-24-2021) | Very clear signals, no nonspecific staining
 |
FH Maurice (Verified Customer) (10-24-2019) | Very clear signals, no undetermined bands like others antibody
|
