PE Anti-Mouse CD357 (GITR) (DTA-1)

CD357 (GITR) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

DTA-1

Cat no : PE-65102

Synonyms

Tnfrsf18, GITR, CD357, AITR

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using PE-65102

  1X10^6 mouse splenocytes were surface stained with 0.06 ug APC-Anti-Mouse CD4 (APC-65104, clone GK1.5), 0.125 ug FITC-Anti-Mouse CD25 (FITC-65137, clone PC61.5) and 0.06 ug PE-Anti-Mouse CD357 (GITR) (PE-65102, clone DTA-1). Cells were not fixed. CD4+ lymphocytes were gated for analysis of CD25 and CD357 (GITR) staining.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65102 targets CD357 (GITR) in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse CD25+ CD4+ T cells
• Full Name: tumor necrosis factor receptor superfamily, member 18
• GenBank Accession Number: BC146517
• Gene Symbol: CD357
• Gene ID (NCBI): 21936
• RRID: AB_2883893
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).