1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR, Clone: 241711C12) or 0.25 ug Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR, Clone: 241711C12) or 0.25 ug Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human PBMCs using 98184-1-RR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR, Clone: 241711C12) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR, Clone: 241711C12) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98184-1-RR targets CD33 in FC applications and shows reactivity with human samples.
Store at 2 - 8°C. Stable for one year after shipment.
Background Information
CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR, Clone: 241711C12) or 0.25 ug Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human PBMCs using 98184-1-RR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD33 Rabbit Recombinant Antibody (98184-1-RR, Clone: 241711C12) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD33 Recombinant antibody
Catalog Number
98184-1-RR
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
